人脑微血管内皮细胞裂解液-上海和序生物科技有限公司

人脑微血管内皮细胞裂解液

更新：2020/5/25 16:29:18 点击：

品牌：人脑微血管内皮细胞裂解液
型号：人脑微血管内皮细胞裂解液

产品介绍

人脑微血管内皮细胞裂解液描述：
Human Brain Microvascular Endothelial Cell Lysate (HBMEC Lysate) is prepared from early passage Human Brain Microvascular Endothelial Cells using a modified RIPA buffer (50 mM Tris-HCl pH 7.2, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.4 mM PMSF, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1 μg/ml pepstatin, 1 mM Na3VO4, 5 mM NaF). Lysate concentration is determined by Beckman Coulter DU 800 spectrophotometer. Lysate is diluted and stored in Storage Buffer (Tris-HCl pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue, 5% -mercaptoethanol). Lysate from ScienCell Research Laboratories is convenient and cost effective for researchers as it eliminates the need to acquire expensive tissues.